Zeiss AxioImager M1
Room B:04 Fluorescence microscopy, South wall, BMIC, Synergy LG.
The Zeiss Axio Imager M1 is a compound, upright, high-performance fluorescence microscope for imaging slide-mounted samples.
The system is equipped with a camera (Zeiss AxioCam HRc) and is capable of brightfield, DIC (Differential Interference Contrast), and fluorescence multichannel imaging using a Colibri 7 LED configurable light source. The fully motorised stage and filter turret is controlled using ZEN software allowing multi-dimensional data acquisition, such as multiple fluorescence channels, tiled images, Z-stacks, and time lapse.
By default the microscope is set up with DAPI, CFP, GFP, YFP and DsRed filter cubes. Additional filters are available and detailed below.
The microscope is equipped with 5x, 10x, 20x and 40x dry objective lenses along with 63x (oil) and 100x (water) immersion objectives.
Please get in touch with us if you are unsure which filter cube/light is best suited to your experiment.
Training: use of this microscope requires at least one training session with Viv or Phil. Please contact us at least 2 weeks in advance to organise training. Registration to book the equipment will not be available until training is complete.
| Filter sets available in BMIC | ||||||||
| 02 (DAPI) | 47 (CFP) | 38 HE | 09 | 46 HE | 46 | 43 HE (DS-RED) | 64 HE (mPlum) | |
| Ex (nm) | 365 | 423-448 | 450-490 | 450-490 | 487-512 | 490-510 | 537-562 | 575-600 |
| Beam splitter | 395 | FT 455 | FT 495 HE | FT 510 | FT 515 HE | FT 515 | FT 570 HE | FT 605 |
| Em (nm) | >420 | 460-500 | 500-550 | >515 | 520-550 | 520-550 | 570-640 | 612-682 |
Image gallery – example images from the AxioImager M1
Phil Hands - Brightfield micrograph of a Canola stem section stained with Toluidine blue dye. Brightfield image of Canola stem section, Toluidine blue stained
DIC image of Fluoresecently labeled yeast cells
Fluoresecent image of maize protoplasts expressing GFP
Phil Hands - Fluorescence microscopy. Tiled, multichannel fluorescence image of a canola flower
Images uses extended depth of focus imaging on the Zeiss AxioImager to capture an image of thick material.
A BMIC user manual and list of BMIC filters is available via the EZbooking object page
Additional resource downloads
Zeiss AxioImager operating manual – Download
Colibri 7 LED light source operating manual – Download