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You can read more about this technology in our peer reviewed manuscripts:

  1. NxtOil version 2 technology results in the accumulation of >35% oil in plant leaves. Step changes in leaf oil accumulation via iterative metabolic engineering. Metab Eng. 2017 39:237-246. doi: 10.1016/j.ymben.2016.12.007.
  2. NxtOil leaf oil profiles can be changed dramatically, including the introduction of medium-chain fatty acids such as C8:0, C10:0, C12:0, C14:0. Thioesterase overexpression in Nicotiana benthamiana leaf increases the fatty acid flux into triacylgycerol. FEBS Lett. 2017 591(2):448-456. doi: 10.1002/1873-3468.12539.
  3. NxtOil technology works in plant tubers, demonstrated here in potato! Genetic enhancement of oil content in potato tuber (Solanum tuberosum L.) through an integrated metabolic engineering strategy. Plant Biotechnol J. 2017 15(1):56-67. doi: 10.1111/pbi.12590.
  4. Version 1 NxtOil technology resulted in the accumulation of 15% oil in plant leaves. Metabolic engineering of biomass for high energy density: oilseed-like triacylglycerol yields from plant leaves. Plant Biotechnol J. 2014 12(2):231-9. doi: 10.1111/pbi.12131.
  5. Demonstration of the initial technology concept which identified the key synergistic response used in NxtOil plants. Synergistic effect of WRI1 and DGAT1 coexpression on triacylglycerol biosynthesis in plants. FEBS Lett. 2013 587(4):364-9. doi: 10.1016/j.febslet.2012.12.018.