Laboratory Analysis
Laboratory analyses of soil will be conducted using recognised methodologies as detailed in the tables below.

Analysis in accordance with the following methodology:
a. the ‘‘Soil Chemical Methods – Australasia’, Rayment GE and Lyons DJ, 2011. CSIRO Publishing, Australia (available at: https://ebooks.publish.csiro.au/content/csirobk/9780643101364/9780643101364.body.pdf; and
b. the ‘Soil Physical Measurement and Interpretation for Land Evaluation. McKenzie N, Couglan K, Cresswell H, 2002. CSIRO Publishing, Australia (available at: Soil Physical Measurement and Interpretation for Land Evaluation | CSIRO Publishing

From about 3,000 sites estimate analysis of 30,000 samples for chemistry and archiving, 6,000 for biology and 600 for physics.
c. Biology eDNA parameters. NSMP are working with Australian Microbiome and Bioplatforms Australia.
Soil sampling methodology follows meth_1.1 Soil Sampling for Biomes of Australian Soil Environments BASE – Australian Microbiome Scientific Manual
Two amplicons are measured: Bacterial Meth_4.1.1 and Fungal Meth_4.1.7 4.1 Library Preparation (incl. amplicon generation where appropriate) – Australian Microbiome Scientific Manual
Soil microbial data has been generated through a collaboration with the National Soil Monitoring Program and the Australian Microbiome Initiative. The National Soil Monitoring Program has been supported by funding through the Australian Government Natural Heritage Trust (Department of Agriculture, Fisheries and Forestry) in collaboration with CSIRO. The Australian Microbiome Initiative is supported by funding from Bioplatforms Australia and the Integrated Marine Observing System (IMOS) enabled by the Commonwealth Government National Collaborative Research Infrastructure Strategy (NCRIS), and CSIRO.