The MIF Myth + post-docs at CSIRO

Timothy Muusse^{1 2}, Sriprada Mahabhashyam^{1 3}, Benjamin Cao^{1 2}, and Jim Harris^{1 4}

¹ Cell Biology Assays, Manufacturing, CSIRO, Clayton, VIC, Australia

² Australian Regenerative Medicine Institute, Monash University, Clayton, VIC, Australia

³ Flinders University, Bedford Park, SA, Australia

⁴ Centre for Inflammatory Diseases, Faculty of Medicine, Nursing and Health Scineces, Monash University, Clayton, VIC, Australia

Topics covered in this poster presentation:

• Immune protein-protein interactions and the role of macrophage migration inhibitory factor (MIF).
• MIF inhibitors induce cell death
• siRNA knock-down to mimic MIF knock-out cells
• Molecular tag knock-in using precision CRISPR editing
• Future directions
• Post-doctoral research at CSIRO

References

Sun, H.W., et al., Crystal structure at 2.6-A resolution of human macrophage migration inhibitory factor. Proc Natl Acad Sci USA, 1996. 93(11): p.5191-6

Zhong, H.N., et al., High-fidelity, efficient, and reversible labeling of endogenous proteins using CRISPR-based designer exon insertion. Elife, 2021, 10.

Harris, J. and N.A. Borg, The multifaceted roles of NLRP3-modulating proteins in virus infection. Front Immunol, 2022. 13: p. 987453.