Viewing Images Using See3
See3 is a webtool that allows you to view, score and optimise crystallisation experiments. To login you will need a username and password (Access C3), and know a few basic things about your experiment (the project, barcodes of the plates and the date they were set up is a good start).
The most basic function ofSee3 allows users to view the images produced from crystallisation experiments. The C3 uses two Formulatrix Rock Imager 1000 imagers. Plates are inspected four times in the first week, twice in the second week, then once a week for a total of 15 inspections (after inspection is complete, the plates are removed).
- After logging in to See3 you’ll be presented with a collapsed tree view of all your projects
- Clicking on a row in the grid will open up the latest inspection for that plate.
- There are a number of options on this view:
- There are three tabs on the upper, left hand side (circled in red, below) – ‘Plates’, ‘Optimisation’ and ‘Clipboard’.
- There are three tabs in the upper, middle screen (circled in green) ‘General’, ‘Plate’, ‘Drop’.
- There are four pulldown menus on the upper, right hand side (circled in purple) ‘Sort by’, ‘Score’, ‘Type’ ‘Subwells’
- There are 6 tabs in the middle of the screen, below the drops (circled in navy blue) ‘Inspections’, ’Conditions’, ’Scoring’, ‘History’, ’Tools’ and ‘Reports’.
UV images are taken at every fifth inspection. To view the UV imaged associated with a plate, expand the the project and barcode of interest until you can see the individual inspections. There is a page discussing how to interpret UV imaging under FAQs.
In C3 we generally collect zoomed colour images collected with visible light. Our imaging schedules include other types of images – for example, images collected with UV light, or with polarising filters in place. If there is more than one type of image collected at the same time (i.e. in the same inspection), they can be accessed by choosing the appropriate image type from the ‘Type’ pulldown. The example below shows that there were both visible and UV images for a particular inspection, and the visible option has been selected.
NEXT: Scoring Images