In order for us to set up your experiment your need to provide us with sufficient sample. The sample calculator (above) provides a good estimate of the amount of sample that is required.
- This is for an individual protein sample, which is to be dispensed into a subwell set (see below). If you have two samples you must supply the calculated amount for each sample.
- There are three fields in the calculator: ‘Number of 96 well plates’, ‘Volume of sample in crystallisation droplet ‘ and ‘Amount of sample required’; the radio button on the right selects which field will be calculated.
- The amount of sample required is the amount we need to complete the experiment, for well-behaved, soluble samples. If your sample is viscous or is precipitating out, you may need to supply more volume.
- The final volume of the droplet will be the volume of the sample + the volume of the reservoir solution
- The calculator is not appropriate to estimate sample requirements for LCP experiments.
- In C3, the 96 well crystallisation plates have two subwells (called, imaginatively, ‘top subwell’ (or ‘subwell 1’), and ‘bottom subwell’ (or ‘subwell 2’)).
- A ‘subwell set’ is all the top subwells (or all the bottom subwells). Thus a subwell set contains 96 subwells. Only one protein can go in a subwell set, so we can have a maximum of two proteins per plate.