To access C3 you need to have a user account. Each user account is under the umbrella of a research group (called ‘Institute’. An Institute agreement last for two years (biennial from Ides of March, 2016) and basically says that you will pay us for the work, and that you own IP coming from the experiments we set up for you.
The easiest way to book a protein sample for screening at the C3. Updated to include our new SHOTGUN design.
Information on how proetases may be applicable to your experiment and how to book this with the C3.
Freezing and thawing protein samples almost certainly results in some degradation of the protein sample, however, for long term storage of purified protein there aren’t many other feasible options. The following is our suggested strategy for snap freezing biological samples.
Most proteins contain tryptophan, which when excited by UV light will fluoresce at between 300-350nm. UV imaging thus allows one to locate protein crystals easily, and to validate that you’ve grown a protein crystal and not a misleading salt crystal.
Information on how to prepare seeds to facilitate nucleation for your crystallisation experiments.