Media Comparison

Concentrations of reagents added to the media used by ANACC
All values in μM AF-6 f2 (CSIRO) GSe (CSIRO) Hypersaline f2 K L1 (NCMA) MBL (CSIRO) MJ MLA PRO99 (NCMA) WC+Si
Base solution used for Medium deionised H2O seawater 80% seawater 130 g/L seasalt seawater seawater deionised H2O seawater deionised H2O seawater deionised H2O
Major nutrients
Nitrate – NaNO3 1647 882 882 882 882 882 3530 2000 1000
Nitrate – KNO3 1978
Ammonium nitrate – NH4NO3 275
Ammonium – NH4Cl 50 101 800
Phosphate – K2HPO4 29 200 40 57 200 50
Phosphate – KH2PO4 73
Phosphate – NaH2PO4.2H2O 36 36
Phosphate – NaH2PO4.H2O 36 50
Na2Glycerophosphate 10
Silicate – Na2SiO3.5H2O 54 54 54 482 482 200
Silicate – Na2SiO3.9H2O 106
NaHCO3 150 208 150
CaCl2.2H2O 68 272 200 250
MgSO4.7H2O 122 162 200 150
Na2SO3 100
Chelators / Buffers
FeCl3.6H2O 4 12 6 1.2
FeNaEDTA 12
EDTA 34
Na2EDTA.2H2O 13 40 81 100 12 12 0.67 12 1.2 12
Ferric citrate 8 18 18 18 14
Citric acid 10 23 23 23 16 17
TRIS 999 1651
MES 2049
Trace Elements
CoCl2.6H2O 0.08 0.05 0.55 0.05 0.046 0.05 0.04 0.06 0.042 0.005 0.042
CuSO4.5H2O 0.04 0.04 0.020 0.010 0.04 0.3 0.040 0.040
H2SeO3 0.010 0.010 0.010 0.010 0.010
H3BO3 554 24.26 40 16
K2CrO4 0.010
MnCl2.4H2O 0.91 0.9 22 0.9 0.910 0.909 0.9 5.05 1.819 0.090 0.910
MnCl2.H2O
Na2MoO4.2H2O 0.05 0.03 0.03 0.026 0.0822 0.03 0.025 0.003 0.025
NH4VO3 0.214
Na3VO4 0.0100
(NH4)6Mo7O24.4H2O 0.012
NiSO4.6H2O 0.0100 0.01
ZnCl2 2.2
ZnSO4.7H2O 0.382 0.077 0.077 0.0765 0.0800 0.0765 0.087 0.0765 0.0080 0.0765
Vitamins
Thiamine 0.0296 0.296 2.96 0.296 0.5930 0.2960 0.5930 0.2965 0.2965
Biotin 0.0082 0.0020 0.0082 0.0020 0.0041 0.00205 0.0041 0.0002 0.0020
B12 0.00074 0.0004 0.00074 0.0004 0.0007 0.000369 0.0007 0.037 0.00004 0.0004
B6 0.0059
Soil Extract Yes