Biospherical QSL100
Using the Biospherical Instruments QSL-100 Laboratory Quantum Scalar Irradiance Meter.
- Open light meter case carefully.
- Check battery by holding on/off switch on “test” and observing meter reading (indicator should be to the far left of the meter scale, past the “bat.” arrow).
- Zero meter if necessary by adjusting “zero” dial.
- Check calibration switch. Flick switch to “dry” for measurements in air; to “wet” for measurements in liquid (e.g. in culture flask filled with distilled water).
- Adjust “full scale irradiance” dial (quanta sec-1 cm-2) to a higher reading than anticipated.
- Carefully handle light probe, especially the spherical sensor on the end. Place sensor in area when light intensity is to be measured.
- Switch on/off switch to “on”. Adjust “full scale irradiance” dial to suitable level for accurate reading.
- Read the appropriate scale for light intensity measurement.
Note that there are two scales on the meter: the top scale from 0-1
and the bottom scale from 0-3. This corresponds to the calibration
factor chosen on the irradiance dial. For example:
- If the irradiance dial is set at 3×1016, then use the bottom scale, such that a meter reading of 2.5 would be recorded as 2.5 x 1016
- If the irradiance dial is set at 1×1015, then use the top scale such that a meter reading of 0.75 would be recorded as 0.75 x1015 …or 7.5 x1014.
- A meter reading of 8.0 on 1×1015 scale and 0.8 on 3×1016 scale are the same measure, ie. 8 x1015
- After measuring light intensity, switch on/off switch to “off’ and carefully replace light probe in light meter case. Dry light probe gently with a tissue if wet. Close light meter case.
To convert quanta cm-2 sec-1 to micromol photons m-2 sec-1, use the formula:
y (micromol photons m-2 sec-1) = quanta cm-2sec-1
6 x 1013
The unit micromol photons m-2 sec-1 is equivalent to the older term microeinsteins m-2 sec-1
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