TABLE 1. Metadata Field Codes Summary

Metadata Field Code

Method Name 

meth_1.1

Soil Sampling for Biomes of Australian Soil Environments BASE

meth_1.2

Soil Sampling for UTAS Urban Soil and Aerial Microbiome Study

meth_1.3

Marine Sediment Sampling for Marine Microbes Coastal Project

meth_1.4

Pelagic Water Sampling for Integrated Marine Observing System National Reference Stations

meth_1.5

Coastal Seawater Sampling for Australian Coastal Microbial Observatory Network

meth_1.5.1

Botany Bay, NSW, and Port Phillip Bay, VIC

meth_1.5.2

Great Barrier Reef, QLD

meth_1.6

Pelagic Water Sampling for Ocean Voyages

meth_1.7

Coastal Seawater Sampling for Downstream DNA Analyses for Marine Microbes Coastal Project

meth_1.8

Coastal Seawater Sampling for Downstream DNA Analyses for Marine Microbes Coastal UPDATED SEPT 2019 TT

meth_1.9

Coastal Seawater Sampling for Downstream RNA Analyses for Marine Microbes Coastal Project

meth_1.10

Coastal Seawater Sampling for Downstream DIC and TA Analyses for Marine Microbes Coastal Project

meth_1.11

Seagrass Sampling, Marine Microbes Coastal

meth_1.11.1

Surficial Sediment Sampling

meth_1.11.2

Seagrass Sampling

meth_1.12

Sponge Sampling for Marine Microbes Coastal Project

meth_1.13

Seaweed Ecklonia radiata Sampling for Marine Microbes Coastal Project

meth_1.14

Coral Sampling for Marine Microbes Coastal Project

meth_1.15

Water Sampling from Acidic Saline Lakes

meth_1.16

Water Sampling from Brisbane River Estuary

meth_1.17

Plant Sampling including leaf root and soil

meth_1.18

Coastal Water Sampling and Filtering for DNA Analysis in the Derwent Estuary

meth_1.19

National Geochemical Survey of Australia Field Manual Geoscience Australia

meth_1.20

Northern Australian Geochemical Survey Geoscience Australia

meth_1.21

Regional geology and mineral systems of the Stavely region in western Victoria Geoscience Australia

meth_2.1

CSBP Laboratory Procedures for Soil and Water Biomes of Australian Soil Environments BASE

meth_2.2

Integrated Marine Observing System National Reference Stations Biogeochemical Operations Manual

meth_2.2int

IMOS sample metadata produced from integrated water column measurements

meth_2.4

CSIRO RV Southern Surveyor Metadata Procedures SS2012_T06

meth_2.5

CSIRO RV Southern Surveyor Metadata Procedures SS2012_T07

meth_2.6

CSIRO RV Southern Surveyor Metadata Procedures SS2012_V04

meth_2.7

CSIRO RV Southern Surveyor Metadata Procedures SS2013_T03

meth_2.8

CSIRO RV Investigator Metadata Procedures IN2014_E03

meth_2.9

CSIRO RV Investigator Metadata Procedures IN2015_C02

meth_2.10

CSIRO RV Investigator Metadata Procedures IN2015_V03

meth_2.11

CSIRO RV Investigator Metadata Procedures IN2016_T01

meth_2.12

CSIRO RV Investigator Metadata Procedures IN2016_V03

meth_2.13

CSIRO RV Investigator Metadata Procedures IN2016_V04

meth_2.14

Sediment Grain Size Fractionation percent fines in marine sediments Marine Microbes Coastal Project

meth_2.15

Sediment Grain Size Fractionation percent fines in marine seagrass sediments Marine Microbes Coastal

meth_2.16

Total Organic Carbon TOC in marine sediment Marine Microbes Coastal Project

meth_2.17

Total Organic Carbon TOC in marine seagrass sediment Marine Microbes Coastal Project

meth_2.18

Porewater nutrients from marine sediment Marine Microbes Coastal Project

meth_2.19

PAM Fluorometry in situ measurements of seagrass Marine Microbes Coastal Project

meth_2.20

Analytical Services Tasmania Laboratory Procedures Derwent Estuary Program, Ambient Water Quality Program

meth_2.21

In situ water measurements using a YSI EXO 3 multiparameter sonde Derwent Estuary Program Ambient Water Quality Program

meth_2.22

CSIRO RV Investigator Laboratory Procedures IN2021_V04

meth_2.23

CSIRO RV Investigator Laboratory Procedures IN2019_V03

meth_2.24

Contextual Metadata for Sediment Sampling in East Coast Australian Estuaries

meth_2.25

AIMS Marine Monitoring Program for Inshore Water Quality QAQC Manual

meth_2.26

Soil visible-near infrared vis-NIR spectra for BASE samples

meth_2.29

Environment Protection Agency Victoria Water Quality Monitoring Methods AM0001

meth_3.1.1

Qiagen DNeasy PowerLyzer PowerSoil Kit for BASE at AGRF

meth_3.1.2

Qiagen DNeasy PowerSoil Kit for soil and rhizosphere and sediments

meth_3.1.3

Qiagen DNeasy PowerSoil modified for marine sediments Marine Microbes Coastal Project

meth_3.1.4

Qiagen DNeasy PowerSoil modified for coastal seawater Marine Microbes Coastal Project

meth_3.1.5

Qiagen DNeasy PowerSoil modified for seagrass Marine Microbes Coastal Projects

meth_3.1.6

MoBio PowerPlant Pro DNA Isolation Kit for coral Marine Microbes Coastal Project

meth_3.1.7

Qiagen DNeasy PowerWater Sterivex Kit

meth_3.1.8

Qiagen DNeasy PowerWater Sterivex Kit modified for removal of filter from sterivex

meth_3.1.9

Qiagen DNeasy PowerWater Sterivex Kit modified IMOS NRS ACMON and voyages CSIRO Hobart Genetics Laboratories

meth_3.1.10

MP Biomedicals FastDNA Spin Kit for Soil modified for soil crust and filtered water samples from acidic saline lakes

meth_3.1.11

Omega BioTek Inc Mag-Bind Plant DNA DS Kit M1130-00

meth_3.2.1

Qiagen RNeasy PowerSoil Total RNA Kit

meth_3.2.2

Qiagen RNeasy PowerSoil Total RNA Kit modified for marine sediments Marine Microbes Coastal Project

meth_3.2.3

PureLink RNA Mini Kit modified for seagrass Marine Microbes Coastal Project

meth_3.2.4

PureLink RNA Mini Kit modified for sponges Marine Microbes Coastal Project

meth_3.3.1

DNA Quantification using NanoDrop Spectrophotometer Thermo Scientific

meth_3.3.2

Fluorometric Nucleic Acid Quantification at AGRF

meth_4.1.1

Bacterial 16S rRNA gene (27F and 519R) 1-step using Immolase™ DNA Polymerase (Bioline)

meth_4.1.2

Bacterial 16S rRNA gene (27F and 519R) 1-step using KAPA HiFi Hot Start ReadyMix (Roche)

meth_4.1.3

Archaeal 16S rRNA gene (A2F and 519R*) 2-step using Immolase™ DNA Polymerase (Bioline)

meth_4.1.4

Eukaryotic 18S v4 rRNA gene (TAReuk454FWD1 and modified TAReukREV3) 1-step

meth_4.1.5

Eukaryotic 18S V9 rRNA gene (1391F and EukBR) 1-step (5 PRIME HotMasterMix)

meth_4.1.6

Fungal ITS gene (ITS1F and ITS4) 1-step

meth_4.1.7

Illumina TruSeq Nano Library Preparation for Metagenomics (BASE)

meth_4.1.8

Illumina Nextera XT Library Preparation for Metagenomics (Marine Microbes 2015-2017)

meth_4.1.9

Metagenomics Library Preparation (Australian Microbiome)

meth_4.1.10

Illumina Ribo-Zero rRNA Removal Kit for Metatranscriptomics (Marine Microbes 2015-2017)

meth_4.1.11

Illumina TruSeq Stranded mRNA Library Preparation for Metatranscriptomics (Marine Microbes 2015-2017)

meth_4.2.1

Illumina MiSeq 2 x 300 bp paired reads

meth_4.2.2

Illumina MiSeq 2 x 250 bp paired reads

meth_4.2.3

Illumina MiSeq 2 x 150 bp paired reads

meth_4.2.4

Illumina HiSeq 2500 Rapid v2 (BASE metagenomics sequencing)

meth_4.2.5

Illumina HiSeq 2500 Rapid SBS 500-cycle v2 (Marine Microbes metagenomics sequencing 2015-2017)

meth_4.2.6

Illumina HiSeq 2500 SBS v4 (Marine Microbes transcriptomics sequencing 2015-2017)

meth_4.2.7

Illumina NovaSeq 6000 (Australian Microbiome 2017 – ongoing)

meth_5.1

Amplicon Analysis