meth_4.2.7 Illumina NovaSeq 6000 (Australian Microbiome 2017 – ongoing)

Contributor(s): Ramaciotti Centre for Genomics, UNSW, Sydney

  1. Denature and dilute the library pool according to the NovaSeq 6000 Denature and Dilute Libraries Guide (1000000106351).
  2. Follow Illumina’s NovaSeq 6000 System Guide (Document # 1000000019358) to prepare NovaSeq reagents for a 2x150bp sequencing run using the Standard workflow when sequencing on a whole flow cell or using the Xp workflow when sequencing in lanes of a flow cell.
  3. Start the NovaSeq 6000 sequencing run according to the system guide referenced above.
  4. After sequencing is complete, perform the base-calling and demultiplexing using bcl2fastq Conversion Software.