meth_4.2.6 Illumina HiSeq 2500 SBS v4 (Marine Microbes Project 2015-2017, transcriptomics sequencing)

Contributor(s): Ramaciotti Centre for Genomics, UNSW, Sydney

1. Denature the 2 nM pool according to the Illumina’s HiSeq and GAIIx Systems -Denature and Dilute Libraries Guide (Document # 15050107 v03, November 2016).
2. Following Illumina’s cBot System Guide (Document # 15006165 v02 January 2016), perform clustering of HiSeq PECluster Kit v4 (PE-401-4001).
3. Following Illumina’s HiSeq 2500 System Guide System Guide (Document # 15035786 v01 October 2015), prepare the HiSeq SBS v4 Reagents for a 2×100 bp PE or 2×125 bp PE sequencing run.
4. Start the HiSeq2500 sequencing run according to the system guide referenced above.
5. After sequencing is complete, perform the basecalling and demultiplexing using bcl2fastq Conversion Software.