meth_4.2.5 Illumina HiSeq 2500 Rapid SBS 500-cycle v2 (Marine Microbes Project 2015-2017, metagenomics sequencing)

Contributor(s): Ramaciotti Centre for Genomics, UNSW, Sydney

  1. Following Illumina’s HiSeq 2500 System Guide (Document # 15035786 v01, October 2015), thaw one HiSeq 2500 Rapid SBS 500-cycle v2 kit per flowcell.
  2. Denature the 2 nM pool according to the Illumina’s HiSeq and GAIIx Systems -Denature and Dilute Libraries Guide (Document # 15050107 v03, November 2016).
  3. Start the HiSeq 2500 Rapid sequencing run according to the system guide referenced above.
  4. After sequencing is complete, perform the basecalling and demultiplexing using bcl2fastq Conversion Software.