meth_4.2.4 Illumina HiSeq 2500 Rapid v2 (BASE metagenomics sequencing)

Contributor(s): Ramaciotti Centre for Genomics, UNSW, Sydney

1. Thaw Illumina’s HiSeq2500 Rapid v2 kits. Use one 200-cycle kit and two 50-cycle kits if performing one run, and three 200-cycle kits if performing two runs simultaneously. Follow Illumina’s HiSeq Rapid SBS Kit v2 Reference Guide (Part# 15058772, Rev. B, May 2015).
2. Denature the 2nM pool according to the Illumina protocol (Denaturing and Diluting Libraries for the HiSeq and GAIIx, Part# 15050107 Rev. C, November 2014).
3. Start the HiSeq 2500 Rapid sequencing run according to the user guide (HiSeq2500 System User Guide, Part# 15035786 Rev. D, November 2014).
4. After sequencing is complete, demultiplex the data using Casava 1.8.2.