meth_4.2.2 Illumina MiSeq 2 x 250 bp paired reads

Contributor(s): Ramaciotti Centre for Genomics, UNSW, Sydney

E.g. 18Sv4

1. Denature according to IIlumina protocol, with increased PhiX control spike as recommended for low diversity libraries. See Preparing Libraries for Sequencing on the MiSeq (part #15039740).
2. Prepare MiSeq Reagent Cartridge (v2 500-cycles). See MiSeq Reagent Kit v2 – Reagent Preparation Guide (part # 15034097).
3. Load 600 μL of library pool into the MiSeq reagent cartridge in designated reservoir/sample well.
4. Modify sample sheet to include appropriate primer’s sequence/indexes.
5. Start sequencing run following MiSeq System User Guide (part # 15027617).