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Contents
Introduction
Contributors
Citations
TABLE 1. Metadata Field Codes Summary
1. Sample Collection Procedures
2. Contextual Metadata Analytical Procedures
3. OMICS Sample Extraction, Purification and Quantification Procedures
4. OMICS Sequencing Procedures
5. Bioinformatics
6. Data Analyses
7. References
4. OMICS Sequencing Procedures
4.1 Library Preparation (incl. amplicon generation where appropriate)
4.2 Next Generation Sequencing
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4. OMICS Sequencing Procedures
4.2 Next Generation Sequencing
4.2 Next Generation Sequencing
meth_4.2.1 Illumina MiSeq 2 x 300 bp paired reads
meth_4.2.2 Illumina MiSeq 2 x 250 bp paired reads
meth_4.2.3 Illumina MiSeq 2 x 150 bp paired reads
meth_4.2.4 Illumina HiSeq 2500 Rapid v2 (BASE metagenomics sequencing)
meth_4.2.5 Illumina HiSeq 2500 Rapid SBS 500-cycle v2 (Marine Microbes Project 2015-2017, metagenomics sequencing)
meth_4.2.6 Illumina HiSeq 2500 SBS v4 (Marine Microbes Project 2015-2017, transcriptomics sequencing)
meth_4.2.7 Illumina NovaSeq 6000 (Australian Microbiome 2017 – ongoing)
