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Contents
Introduction
Contributors
Citations
TABLE 1. Metadata Field Codes Summary
1. Sample Collection Procedures
2. Contextual Metadata Analytical Procedures
3. OMICS Sample Extraction, Purification and Quantification Procedures
4. OMICS Sequencing Procedures
5. Bioinformatics
6. Data Analyses
7. References
4. OMICS Sequencing Procedures
4.1 Library Preparation (incl. amplicon generation where appropriate)
4.2 Next Generation Sequencing
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4. OMICS Sequencing Procedures
4.1 Library Preparation (incl. amplicon generation where appropriate)
4.1 Library Preparation (incl. amplicon generation where appropriate)
meth_4.1.1 Bacterial 16S rRNA gene (27F and 519R) 1-step using Immolase™ DNA Polymerase (Bioline)
meth_4.1.2 Bacterial 16S rRNA gene (27F and 519R) 1-step using KAPA HiFi Hot Start ReadyMix (Roche)
meth_4.1.3 Archaeal 16S rRNA gene (A2F and 519R*) 2-step using Immolase™ DNA Polymerase (Bioline)
meth_4.1.4 Eukaryotic 18S v4 rRNA gene (TAReuk454FWD1 and modified TAReukREV3) 1-step
meth_4.1.5 Eukaryotic 18S V9 rRNA gene (1391F and EukBR) 1-step (5 PRIME HotMasterMix)
meth_4.1.6 Fungal ITS gene (ITS1F and ITS4) 1-step
meth_4.1.7 Illumina TruSeq Nano Library Preparation for Metagenomics (BASE)
meth_4.1.8 Illumina Nextera XT Library Preparation for Metagenomics (Marine Microbes Project 2015-2017)
meth_4.1.9 Metagenomics Library Preparation (Australian Microbiome 2017-ongoing)
meth_4.1.10 Illumina Ribo-Zero rRNA Removal Kit for Metatranscriptomics (Marine Microbes Project 2015-2017)
meth_4.1.11 Illumina TruSeq Stranded mRNA Library Preparation for Metatranscriptomics (Marine Microbes Project 2015-2017)
