meth_3.1.4 Qiagen DNeasy PowerSoil modified for coastal seawater Marine Microbes Coastal Project

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AM Project ID: Legacy

  1. For DNA extraction, above a clean surface (e.g., petri dish) crack open the sterivex filter at the inlet end using long-handled pincers (e.g., Oetiker-14100329-Economy-Tool-Standard-Jaw-Pincers)
  2. The PES membrane can be carefully removed by scoring along the edges with a pair of clean watch-makers forceps and peeling off its support and before placing the membrane into an extraction tube.
  3. Extract using the Qiagen DNeasy® PowerSoil® Kit, which includes a beating step with the vortex adaptor.
  4. The final eluted DNA should be labelled according to the instruction given in the spreadsheet “sampling_schedule” and stored at -80 °C.