meth_3.1.12 Qiagen DNeasy PowerSoil Kit for marine sponges

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Sponge:

1. Cut all three piece of each specimen (n=3) into small pieces under liquid nitrogen. Mix the small pieces for each specimen, but don’t mix pieces of the sponge replicates.
2. Extract frozen samples (~0.5 g) for each replicate (n=3) separately with Qiagen DNeasy® PowerSoil® Kit (https://www.qiagen.com/au/products/dneasy-powersoil-kit/#orderinginformation).
3. The final eluted DNA should be labelled according to the instruction given in the spreadsheet “sampling_schedule” and stored at -80 °C.