meth_3.1.1 Qiagen DNeasy PowerLyzer PowerSoil Kit for BASE at AGRF

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AM Project ID: AM_BASE

  1. DNA extraction is carried out in triplicate using the DNeasy PowerLyzer PowerSoil® Kit (Cat No./ID:12855, https://www.qiagen.com/au/products/discovery-and-translational-research/dna-rna-purification/dna-purification/microbial-dna/dneasy-powerlyzer-powersoil-kit/#orderinginformation). The DNA is extracted according to the Earth Microbiome protocol http://www.earthmicrobiome.org/emp-standard-protocols/dna-extraction-protocol/
  2. A soil sample input of 250 mg is used for each extraction
  3. For consistency if added DNA output is required an increased number of extractions will be performed and pooled and noted in the metadata (rather than increasing the soil input into an extraction)
  4. DNA replicate extractions are pooled for further QC and sequencing analysis