meth_2.24 Contextual Metadata for Sediment Sampling in East Coast Australian Estuaries

Contributor(s): Katie Dafforn (katherine.dafforn@mq.edu.au)

Citation: Filippini, Giulia, Ana B. Bugnot, Emma L. Johnston, Jason Ruszczyk, Jaimie Potts, Peter Scanes, Angus Ferguson, Martin Ostrowski, Deepa Varkey, and Katherine A. Dafforn. (2019). Sediment bacterial communities associated with environmental factors in Intermittently Closed and Open Lakes and Lagoons (ICOLLs). Science of The Total Environment, 693, 133462. doi:https://doi.org/10.1016/j.scitotenv.2019.07.268

AM Project ID: AM0014

Temperature

Temperature was measured with a water quality probe (Hydrolab Multimeter).

Bottom depth

Bottom depth was determined through a deep sounder on the boat used for sample collection.

Nutrients analysis

Analysed through Australian Laboratory Services (ALS) environmental testing https://www.alsglobal.com/en-au/

Total organic Carbon

ALS standard method EP003 (TOC)

Dried and pulverised sample is reacted with acid to remove inorganic Carbonates, then combusted in a LECO furnace in the presence of strong oxidants / catalysts. The evolved (Organic) Carbon (as CO₂) is automatically measured by infra-red detector.

Total Nitrogen

ALS standard method EK062G (TN)

Total nitrogen as N (TKN+NOx) by discrete analyser (APHA 21^st Ed, 4500 Norg/NO3)

Total phosphorus

ALS standard method EK067G (TP)

TP by discrete analyser (APHA 21^st Ed, 4500 P-B&F)

Organic matter content

Organic matter content was determined using the Loss-on-ignition method.

Percentage of sediment organic carbon was measured by loss on ignition (LOI) method. Samples were initially dried at 105 °C for 12 h to remove moisture and then organic matter burned in the muffle furnace at 500 °C for 12 h (Wang et al., 2011).