meth_1.5 Coastal Seawater Sampling for Australian Coastal Microbial Observatory Network
Contributor(s): Murray, S. (Shauna.Murray@uts.edu.au), Sherman, C. (craig.sherman@deakin.edu.au), Thomas, T. (t.thomas@unsw.edu.au), Webster, N (Nicole.Webster@awe.gov.au) and Seymour, J (justin.seymour@uts.edu.au).
Citation:
AM Project ID: AM0001
Standard operating procedures (SOP) for coastal sampling
These SOPs were produced to describe the steps involved in a) sampling microbial communities b) obtaining relevant metadata and c) processing samples for subsequent sequence analysis as part of the marine microbiology project support by Bioplatforms Australia.
General comments:
For each sampling event request three unique BPA IDs.
For each sampling event, please measure and note the following parameters:
- Longitude and latitude of sampling site in decimal degrees
- Time and data in the format dd:mm:yyyy hh:mm
- Depth in metres
Equipment list
- Water sampler or 20 L bucket
- 50 mL syringes
- 45µm syringe filter (e.g. Millex HV Syringe Filter Unit; for nutrients)
- 30 mL nutrient tubes (supplied by CSIRO for nutrient samples)
- Syringe filter holder with 25 mm Whatmann GF/F filter (for chlorophyll sampling)
- Cryovials
- Cryovial with paraformaldehyde (for cell counts)
- Pipette and tips (for 1 mL of seawater for cell counts)
- Dry shipper for liquid N2 (to freeze samples for cell counts)
- Container with screw lid for disposal of pipette tips
- Sterivex filters (for microbial sampling)
- Peristaltic pump system with pre-filter (a 15 Falcon tube with a 100 micron mesh over the threaded part of the lid)
- Data sheets
- Labelling markers
- Ziplock bags